• KN-62: Applied CaMKII Inhibition Workflows

  2026-08-23

  KN-62 enables controlled interrogation of CaMKII in calcium signaling, secretion, metabolism, and proliferation assays. This workflow-focused guide connects biochemical inhibition with cell-based readouts while showing how to distinguish direct CaMKII effects from broader calcium-stress phenotypes.

• NEIL1, COL17A1, and Colorectal Cancer Initiation

  2026-08-22

  Cao et al. identify NEIL1 as a transcriptional regulator that links oxidative DNA-repair biology to colorectal cancer initiation through a SATB2/c-Myc/RNAPII complex and COL17A1 activation. The study combines human samples, genetically modified mice, molecular assays, and immune analyses to show that NEIL1 inhibition may restrain tumor formation and improve cytotoxic T-cell activity.

• Dynasore Workflows for Endocytosis and CRC Models

  2026-08-22

  Dynasore provides reversible, cell-permeable control of dynamin-dependent membrane scission for uptake, trafficking, and signaling experiments. This guide translates that capability into practical endocytosis research and a hypothesis-testing workflow for Fusobacterium nucleatum extracellular vesicles in colorectal cancer models.

• 2X Taq PCR Master Mix for C. elegans Assays

  2026-08-21

  Translate pheromone-neurodegeneration findings into practical C. elegans genotyping, transgene verification, and TA-cloning workflows. This ready-to-use mix combines direct gel loading with Taq-generated adenine overhangs, reducing handling steps while preserving flexible endpoint PCR design.

• Dextromethorphan Hydrobromide: Evidence to Assay

  2026-08-20

  Dextromethorphan hydrobromide is a mechanistically informative NMDA receptor antagonist for studying excitotoxicity, ion-channel modulation, and neuroprotection. This article develops an evidence-triangulation framework that connects assay design with translational interpretation without overstating preclinical findings.

• Cytosolic DNA and TDP-43 Homeostasis

  2026-08-20

  A 2024 Journal of Biological Chemistry study identifies cytosolic DNA accumulation as a regulator of TDP-43 condensation, nuclear depletion, and short-isoform production. Its experiments connect extracellular DNA uptake, DNA damage, and impaired nuclear-envelope integrity to mechanisms relevant to ALS and other TDP-43 proteinopathies.

• Viruses, FPN1, and Iron-Dependent Immune Escape

  2026-08-19

  The reference study identifies a viral immune-evasion mechanism in which DTX3L-driven degradation of ferroportin increases intracellular ferrous iron and weakens TBK1-, STING-, interferon-, and autophagy-dependent antiviral defense. Its genetic, biochemical, and in vivo evidence positions FPN1-dependent iron withholding as an active component of innate immunity rather than only a metabolic process.

• KN-62 and the Calcium Logic of Memory Maintenance

  2026-08-19

  A translational framework for using KN-62 to test whether calcium-sensitive CaMKII signaling links neuroligin 1 cleavage to synaptic remodeling and short-term social memory maintenance, while also positioning the compound across secretion, metabolism, and cell-cycle research.

• KLF4 and AgRP Isoform Control in Mice

  2026-08-18

  Ritter and colleagues show that KLF4 regulates AgRP transcription in a cell- and metabolic-state-dependent manner, with the Agrp-A isoform accounting for most hypothalamic Agrp expression in lean mice. Their combination of isoform-resolved analysis, conditional Klf4 deletion, metabolic phenotyping, and ERK perturbation links transcriptional control to energy expenditure while demonstrating that diet-induced obesity can override this mechanism.

• Bestatin, Actinonin, and Intracellular Myeloma Inhibition

  2026-08-18

  The reference study separates inhibition of cell-surface aminopeptidases from the antiproliferative effects of bestatin and actinonin in U937 and K562 cells. Its transporter-modifier experiments support an intracellular mechanism in which MRP and, for bestatin in K562 cells, P-glycoprotein influence drug accumulation and apparent potency.

• Mdivi-1 Workflow for DRP1 and Apoptosis Research

  2026-08-17

  Mdivi-1 provides a practical pharmacological entry point for testing how DRP1-dependent mitochondrial fission shapes apoptosis, inflammasome signaling, and cell survival. This workflow connects quantitative mitochondrial imaging with apoptosis assays and disease-relevant models, while emphasizing formulation controls and orthogonal validation.

• Mdivi-1: Reframing DRP1 for Translation

  2026-08-17

  Mdivi-1 connects mitochondrial fission, apoptosis, and tissue injury. This thought-leadership analysis interprets recent chronic intermittent hypoxia findings and outlines how a selective DRP1 inhibitor can strengthen mechanistic and translational study design.

• Arachidonic Acid Supplementation and Humoral Immunity

  2026-08-16

  The reference study shows that dietary arachidonic acid can accelerate and strengthen rabies vaccine-induced neutralizing antibody responses in mice and human volunteers. Its mechanistic contribution is the identification of a lymph-node ARA–PGI2–cAMP/PKA pathway connecting lipid metabolism with CD86 expression, AID activation, and germinal-center B-cell maturation.

• Thioguanine Workflows for Cancer and EV71 Studies

  2026-08-15

  Thioguanine provides a practical bridge between thiopurine biology, cancer-cell assays, antiviral screening, and epigenetic studies. This workflow separates direct cytotoxicity from EV71 virus inhibition while using orthogonal controls and the reference study’s lessons in assay validation.

• Verapamil HCl as a Calcium-Flux Assay Tool

  2026-08-14

  Verapamil HCl is more than an L-type calcium channel blocker: it can serve as a controlled perturbation for separating calcium signaling, intracellular drug exposure, apoptosis, and inflammation. This article connects transporter-aware assay design with myeloma and arthritis models while clarifying what verapamil can—and cannot—prove mechanistically.

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